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Selected Publications


  • van Marrewijk LM, Polyak SW, Hijnen M, Kuruvilla D, Chang MR, Shin Y, Kamenecka TM, Griffin PR, Bruning JB. "SR2067 Reveals a Unique Kinetic and Structural Signature for PPARγ Partial Agonism." ACS Chem Biol. 2016 Jan 15;11(1):273-83.


  • Kroker, AJ and Bruning, JB. "Review of the Structural and Dynamic Mechanisms of PPARγ Partial Agonism." PPAR Research. 
  • Coleman, T, Chao, RR, Bruning, JB, De Voss, JJ, Bell, SG. "CYP199A4 catalyses the efficient demethylation and demethenylation of para-substituted benzoic acid derivatives." RSC Advances. 5 (64), 52007-52018.
  • Kojetin DJ, Matta-Camacho E, Hughes TS, Srinivasan S, Nwachukwu JC, Cavett V, Nowak J, Chalmers MJ, Marciano DP, Kamenecka TM, Shulman AI, Rance M, Griffin PR, Bruning JB, Nettles KW. "Structural mechanism for signal transduction in RXR nuclear receptor heterodimers." Nature Communications. 2015 Aug 20;6:8013.
  • Marciano DP, Kuruvilla DS, Boregowda SV, Asteian A, Hughes TS, Garcia-Ordonez R, Corzo CA, Khan TM, Novick SJ, Park H, Kojetin DJ, Phinney DG, Bruning JB, Kamenecka TM, Griffin PR. "Pharmacological repression of PPARγ promotes osteogenesis." Nature Communications. 2015 Jun 12;6:7443.
  • Kroker AJ, Bruning JB. "p21 Exploits Residue Tyr151 as a Tether for High-Affinity PCNA Binding." Biochemistry. 2015 Jun 9;54(22):3483-93.
  • Jia Y, Wong DC, Sweetman C, Bruning JB, Ford CM. "New insights into the evolutionary history of plant sorbitol dehydrogenase." BMC Plant Biology. 2015 Apr 12;15:101.


  • Reddy MC, Breda A, Bruning JB, Sherekar M, Valluru S, Thurman C, Ehrenfeld H, Sacchettini JC. "Structure, activity, and inhibition of the Carboxyltransferase β-subunit of acetyl coenzyme A carboxylase (AccD6) from Mycobacterium tuberculosis." Antimicrobial Agents & Chemotherapy. 2014 Oct;58(10):6122-32.
  • Chua KC, Pietsch M, Zhang X, Hautmann S, Chan HY, Bruning JB, Gütschow M, Abell AD. "Macrocyclic protease inhibitors with reduced peptide character." Angewandte Chemie International Edition. 2014 Jul 21;53(30):7828-31.
  • Sullivan AE, Raimondo A, Schwab TA, Bruning JB, Froguel P, Farooqi IS, Peet DJ, Whitelaw ML. "Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2." Biochemical Jornal. 2014 Aug 1;461(3):403-12.
  • Bersten DC, Bruning JB, Peet DJ, Whitelaw ML. "Human variants in the neuronal basic helix-loop-helix/Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function." PLoS One. 2014 Jan 17;9(1):e85768. 


  • Bonnefond A, Raimondo A, Stutzmann F, Ghoussaini M, Ramachandrappa S, Bersten DC, Durand E, Vatin V, Balkau B, Lantieri O, Raverdy V, Pattou F, Van Hul W, Van Gaal L, Peet DJ, Weill J, Miller JL, Horber F, Goldstone AP, Driscoll DJ, Bruning JB, Meyre D, Whitelaw ML, Froguel P. "Loss-of-function mutations in SIM1 contribute to obesity and Prader-Willi-like features." Journal of Clinical Investigation. 2013 Jul;123(7):3037-41.     
  • Ramachandrappa S, Raimondo A, Cali AM, Keogh JM, Henning E, Saeed S, Thompson A, Garg S, Bochukova EG, Brage S, Trowse V, Wheeler E, Sullivan AE, Dattani M, Clayton PE, Datta V, Bruning JB, Wareham NJ, O'Rahilly S, Peet DJ, Barroso I, Whitelaw ML, Farooqi IS. "Rare variants in single-minded 1 (SIM1) are associated with severe obesity." Journal of Clinical Investigation. 2013 Jul;123(7):3042-50.
  • Mosser R, Reddy MC, Bruning JB, Sacchettini JC, Reinhart GD. "Redefining the role of the quaternary shift in Bacillus stearothermophilus phosphofructokinase." Biochemistry. 2013 Aug 13;52(32):5421-9.


  • Mosser, R, Reddy, MC, Bruning, JB, Sacchettini, JC, Reinhart, GD. "Structure of the apo form of Bacillus Stearothermophilus Phosphofructokinase." Biochemistry. 2012 Jan 11.


  • Choi, JH, Bank, AS, Kamenecka,TM, Busby, SA, Chalmers, MJ, Kumar, N, Kuruvilla, DR, Shin, Y, He, Y, Bruning, JB, Marciano, D, Cameron, MD, Laznik,D, Jurczak, MJ, Schürer, SC, Vidovi, D, Shulman, GI, Spiegelman, BM, Griffin, PR. "Potent Anti-Diabetic Actions of a Non-Agonist PPARγ Ligand that Blocks Cdk5-Mediated Phosphorylation." Nature. 2011 Sep 4.
  • Zhang, J, Chalmers, MJ, Stayrook, KR, Burris, LL, Wang, Y, Busby, SA, Pascal, BD, Garcia-Ordonez, RD, Bruning, JB, Dodge, JA, Burris, TP and Griffin, PR. "DNA binding alters coactivator interaction surfaces of the intact VDR-RXR complex." Nature Structural & Molecular Biology. 2011 May;18(5):556-63.
  • Chim, N, Habel, JE, Johnston, JM, Krieger, I, Miallau, L, Sankaranarayanan R, Morse, RP, Bruning, J, Swanson, S, Kim, H, Kim, CY, Li, H, Bulloch, EM, Payne, RJ, Manos-Turvey, A, Hung, LW, Baker, EN, Lott, JS, James, MN, Terwilliger, TC, Eisenberg, DS, Sacchettini, JC, Goulding, CW. "The TB structural genomics consortium: a decade of progress." Tuberculosis. 2011 Mar;91(2):155-72.
  • Bruning, J.B. , Murillo, AC, Chacon, O, Barletta, RG, and Sacchettini, JC. "Structure of the Mycobacterium tuberculosis D-alanine:D-alanine Ligase: a target of the anti-tuberculosis drug D-cycloserine." Antimicrobial Agents & Chemotherapy. 2011 Jan;55(1):291-301.


  • Bruning, JB, Parent, AA, Gil, G, Zhao, M, Nowak, J, Pace, MC, Smith, CL, Afonine, PV, Adams, PD, Katzenellenbogen, JA, and Nettles, KW. "Coupling of Receptor Conformation and Ligand Orientation Determine Graded Activity." Nature Chemical Biology. 2010 Nov;6(11):837-43.
  • Mosser, R, Reddy, M, Bruning, J, Sacchettini, JC, Reinhart, GD. "Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase ." Biophysical Journal, Volume 98, Issue 3, Supplement 1, January 2010, Page 39a.


  • Reddy MC*, Palaninathan SK*, Bruning JB* , Thurman C, Smith D, Sacchettini JC. "Structural insights into the mechanism of the allosteric transitions of Mycobacterium Tuberculosis cAMP receptor protein." The Journal of Biological Chemistry. 2009 Dec 25;284(52):36581-91.  *Authors contributed equally


  • Dai, SY, Chalmers, MJ, Bruning, J, Bramlett, KS, Osborne, HE, Montrose-Rafizadeh, C, Barr, RJ, Wang, Y, Wang, M, Burris, TP, Dodge, JA, Griffin, PR. "Prediction of the tissue-specificity of selective estrogen receptor modulators by using a single biochemical method." Proceedings of the National Academy of Sciences, USA. 2008 May 20;105(20):7171-6.
  • Nettles, KW*, Bruning, JB* , Gil, G, Nowak, J, Sharma, SK, Hahm, JB, Kulp, K, Hochberg, RB, Zhou, H, Katzenellenbogen, JA, Katzenellenbogen, BS, Kim, Y, Joachmiak, A, Greene, GL. "NFκB selectivity of estrogen receptor ligands revealed by comparative crystallographic analyses" Nature Chemical Biology. 2008 Apr;4(4):241-7.   *Authors contributed equally


  • Bruning, JB , Chalmers, MJ, Prasad, S, Busby, SA, Kamenecka, TM, He, Y, Nettles, KW, Griffin, PR. "Partial agonists activate PPARgamma using a helix 12 independent mechanism." Structure. 2007 Oct;15(10):1258-71.
  • Zhou, HB, Nettles, KW, Bruning, JB , Kim, Y, Joachimiak, A, Sharma, S, Carlson, KE, Stossi, F, Katzenellenbogen, BS, Greene, GL, Katzenellenbogen, JA. "Elemental isomerism: a boron-nitrogen surrogate for a carbon-carbon double bond increases the chemical diversity of estrogen receptor ligands." Chemistry & Biology. 2007 Jun;14(6):659-69.
  • Nettles, KW, Bruning, JB , Gil G, O'Neill, EE, Nowak, J, Hughs, A, Ki, Y, DeSombre, ER, Dilis, R, Hanson, RN, Joachimiak, A & Greene, GL. "Structural plasticity in the oestrogen receptor ligand-binding domain." EMBO Reports. 2007 Jun;8(6):563-8.
  • Chalmers, MJ, Busby, SA, Prasad, S, Bruning, JB , Pascal, BD, Southern, MR, and Griffin, PR. "P97-S A High-Throughput Method for the characterization of Nuclear Receptor Dynamics." Journal of Biomolecular Techniques. 2007 February; 18(1): 33.


  • Bruning, JB and Shamoo, Y. "Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-δ p66 subunit and flap endonuclease-1 (FEN1)." Structure. 2004 Dec;12(12):2209-19.
Protein Crystallography Laboratory

North Terrace Campus
Molecular Life Sciences
The University of Adelaide


Dr John Bruning
T: +61 8 8313 5352
F: +61 8 8313 4362